Coding
Part:BBa_K726016
Designed by: Verna Huang and Helena Viets Group: iGEM12_UCSF (2012-10-02)
T7Prom+HisTag+VioABE
The first three genes in the violacein operon, under the control of a T7 promoter with a lac operator and ribosome binding site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1147
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 123
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3929
Illegal AgeI site found at 4125 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
[edit]
Categories
Parameters
None |